xov xwm

I. Kev khaws cia thiab xa khoom ntawm Bio-pharmaceuticals

(1) Cov tshuaj tiv thaiv muaj kev cuam tshuam rau lub teeb thiab kub thiab txo lawv cov txiaj ntsig sai, yog li lawv yuav tsum tau khaws cia hauv tub yees ntawm 2 txog 5 ° C.Kev ua tsis tiav ntawm cov tshuaj tiv thaiv xws li khov khov muaj qhov cuam tshuam tsis zoo rau kev ua tau zoo, yog li lub tub yees tsis tuaj yeem txias dhau, ua rau cov tshuaj tiv thaiv khov thiab ua tsis tiav.

(2) Thaum cov tshuaj tiv thaiv xa tuaj, nws tseem yuav tsum tau khaws cia rau hauv lub tub yees, thauj los ntawm lub tsheb tub yees, thiab txo lub sij hawm xa khoom sai li sai tau.Tom qab mus txog qhov chaw, nws yuav tsum muab tso rau hauv lub tub yees 4 ° C.Yog tias tsis muaj lub tsheb tub yees tuaj yeem thauj tau, nws yuav tsum tau thauj mus los siv cov yas ntim khoom khov (cov tshuaj tiv thaiv ua kua) lossis dej khov qhuav (cov tshuaj tiv thaiv qhuav).

(3) Cov tshuaj tiv thaiv cell-dependent, xws li cov tshuaj tiv thaiv ua kua rau qaib ntxhw-herpesvirus ntawm Marek tshuaj tiv thaiv, yuav tsum tau khaws cia hauv cov kua nitrogen ntawm qhov kub ntawm 195 ° C.Thaum lub sij hawm cia, xyuas seb cov kua nitrogen hauv lub thawv puas yuav ploj mus txhua lub lim tiam.Yog hais tias nws yuav ploj mus, nws yuav tsum tau ntxiv.

(4) Txawm hais tias lub teb chaws pom zoo cov tshuaj tiv thaiv, yog tias nws tsis raug muab khaws cia, thauj thiab siv, nws yuav cuam tshuam rau qhov zoo ntawm cov tshuaj tiv thaiv thiab txo nws cov txiaj ntsig.

Qhov thib ob, kev siv tshuaj tiv thaiv yuav tsum tau saib xyuas cov teeb meem

(1) Ua ntej tshaj plaws, yuav tsum tau nyeem cov lus qhia siv los ntawm lub Hoobkas tshuaj, thiab raws li nws cov kev siv thiab ntau npaum li cas.

(2) Xyuas seb lub raj mis txhaj tshuaj puas muaj daim ntawv pov thawj tshuaj xyuas thiab seb nws puas dhau hnub tas sijhawm.Yog tias nws tau dhau hnub kawg ntawm cov tshuaj tiv thaiv, nws siv tsis tau.

(3) Cov tshuaj tiv thaiv yuav tsum tsis txhob raug tshav ntuj ncaj qha.

(4) Lub syringe yuav tsum tau boiled los yog chav autoclaved thiab yuav tsum tsis txhob muaj tshuaj tua kab mob (cawv, stearic acid, thiab lwm yam).

(5) Cov tshuaj tiv thaiv qhuav tom qab ntxiv cov tshuaj diluted yuav tsum tau siv sai li sai tau thiab yuav tsum tau siv li ntawm 24 teev ntawm qhov tseeb.

(6) Cov tshuaj tiv thaiv yuav tsum tau siv rau hauv cov tsiaj noj qab haus huv.Kev txhaj tshuaj tiv thaiv yuav tsum raug ncua yog tias tsis muaj zog, tsis qab los noj mov, ua npaws, raws plab, lossis lwm yam tsos mob.Txwv tsis pub, tsis tsuas yog tsis tuaj yeem tau txais kev tiv thaiv zoo, thiab yuav ua rau nws mob ntxiv.

(7) Inactivated vaccine Feem ntau cov adjuvants ntxiv, tshwj xeeb tshaj yog cov roj yooj yim dua los nag.Txhua zaus cov tshuaj tiv thaiv tawm ntawm lub syringe, lub raj mis txhaj tshuaj tau shaken ceev thiab cov ntsiab lus ntawm cov tshuaj tiv thaiv yog homogenized tag nrho ua ntej siv.

(8) Cov tshuaj tiv thaiv lub raj mis thiab cov tshuaj tiv thaiv tsis siv yuav tsum tau muab tshuaj tua kab mob thiab muab pov tseg.

(9) Sau kom meej txog hom tshuaj tiv thaiv siv, lub npe hom, batch naj npawb, hnub tas sij hawm, hnub txhaj tshuaj, thiab txhaj tshuaj, thiab khaws cia rau yav tom ntej siv.

Thib peb, kev txhaj tshuaj tiv thaiv qaib dej haus yuav tsum tau saib xyuas cov teeb meem

(1) Cov dej haus yuav tsum yog cov dej huv uas tsis muaj tshuaj tua kab mob tom qab siv.

(2) Cov tshuaj tiv thaiv diluted yuav tsum tsis txhob tsim cov dej uas muaj cov tshuaj tua kab mob lossis cov kua qaub lossis cov dej alkaline.Cov dej distilled yuav tsum tau siv.Yog tias koj yuav tsum siv cov dej kais dej, ntxiv li 0.01 grams Hypo (Sodium thiosulfate) rau 1,000 ml ntawm cov kais dej tom qab tshem cov kais dej los ntxuav cov kais dej, lossis siv 1 hmo.

(3) Cov dej haus yuav tsum tau ncua ua ntej inoculation, li 1 teev nyob rau hauv lub caij ntuj sov thiab txog 2 teev nyob rau lub caij ntuj no.Nyob rau lub caij ntuj sov, qhov kub ntawm cov dev mub dawb yog qhov siab.Txhawm rau txo qhov poob ntawm cov tshuaj tiv thaiv kab mob, nws raug nquahu kom siv cov dej haus inoculation thaum qhov kub thiab txias thaum sawv ntxov.

(4) Tus nqi ntawm cov dej haus hauv cov tshuaj tiv thaiv yog nyob rau hauv 2 teev.Tus nqi ntawm cov dej haus ib apple ib hnub yog raws li nram no: 4 hnub ntawm hnub nyoog 3 ˉ 5 ml 4 lub lis piam ntawm hnub nyoog 30 ml 4 lub hlis ntawm hnub nyoog 50 ml.

(5) Haus dej ib 1,000 ml Ntxiv 2-4 grams ntawm skimmed mis nyuj hmoov los tiv thaiv cov tshuaj tiv thaiv kab mob ciaj sia.

(6) Yuav tsum npaj cov chaw haus dej kom txaus.Yam tsawg kawg 2/3 ntawm cov qaib hauv ib pawg qaib tuaj yeem haus dej tib lub sijhawm thiab nyob rau lub sijhawm tsim nyog thiab nyob deb.

(7) Cov dej haus tshuaj tua kab mob yuav tsum tsis txhob muab ntxiv rau hauv cov dej haus tsis pub dhau 24 teev tom qab noj cov dej haus.Vim yog cuam tshuam qhov kev loj hlob ntawm cov tshuaj tiv thaiv kab mob hauv qaib.

(8) Txoj kev no yooj yim dua thiab txuag nyiaj ntau dua li kev txhaj tshuaj lossis qhov muag poob, qhov ntswg qhov ntswg, tab sis tsis sib xws ntawm cov tshuaj tiv thaiv kab mob yog nws qhov tsis zoo.

Table 1 Dilution haus muaj peev xwm haus dej qaib hnub nyoog 4 hnub nyoog 14 hnub muaj hnub nyoog 28 hnub muaj hnub nyoog 21 hlis Dissolve 1,000 koob tshuaj dej haus 5 litres 10 litres 20 litres 40 litres Lus Cim: Nws tuaj yeem nce lossis txo raws caij nyoog.Plaub, nqaij qaib tsuag inoculation yuav tsum tau them sai sai rau qhov teeb meem

(1) tshuaj tsuag inoculation yuav tsum tau xaiv los ntawm cov nqaij qaib huv si yog vim qhov kev siv ntawm cov nqaij qaib noj qab haus huv, vim txoj kev no piv nrog lub qhov muag, qhov ntswg thiab txoj kev haus dej, muaj ib tug mob ua pa incursions, Yog hais tias kev txom nyem los ntawm CRD yuav ua rau. CRD phem dua.Tom qab cov tshuaj tsuag inoculation, nws yuav tsum tau khaws cia nyob rau hauv zoo huv si tswj.

(2) npua inoculated los ntawm kev txau yuav tsum muaj 4 lub lis piam los yog laus dua thiab yuav tsum tau thawj zaug los ntawm ib tug neeg uas tau txhaj tshuaj tiv thaiv nyob rau hauv uas tsis tshua muaj siv tshuaj tiv thaiv.

(3) Dilutions yuav tsum muab tso rau hauv lub tub yees 1 hnub ua ntej inoculation.Rau 1,000 ntsiav tshuaj ntawm dilution tau siv nyob rau hauv tawb ntawm 30 ml thiab ca feeders ntawm 60 ml.

(4) Thaum cov tshuaj tsuag yog inoculated, lub qhov rais, qhov cua kiv cua, thiab qhov cua qhov yuav tsum raug kaw thiab ib lub ces kaum ntawm lub tsev yuav tsum mus txog.Nws yog qhov zoo dua los npog cov ntaub yas.

(5) Cov neeg ua haujlwm yuav tsum hnav lub qhov ncauj qhov ntswg thiab tsom iav tsis pom kev.

(6) Txhawm rau tiv thaiv kab mob ua pa, siv tshuaj tua kab mob ua ntej thiab tom qab txau.

Thib tsib, kev siv qaib hauv kev siv tshuaj tiv thaiv

(1) Newtown qaib quail tshuaj tiv thaiv tuaj yeem muab faib ua cov tshuaj tiv thaiv nyob thiab cov tshuaj tiv thaiv tsis muaj zog.