xov xwm

I. Kev Tiv Thaiv thiab Xa Tawm ntawm Bio-pharmaceuticals

(1) Cov tshuaj tiv thaiv yog cov raug rau lub teeb thiab qhov kub thiab txo lawv qhov ua tau zoo, yog li lawv yuav tsum khaws cia hauv tub yees li 2 txog 5 ° C. Ua tsis tiav kev txhaj tshuaj tiv thaiv xws li khov yog qhov tsis zoo rau qhov ua tau zoo, yog li ntawd lub tub yees tsis tuaj yeem txias dhau, ua rau cov tshuaj tiv thaiv khov thiab tsis ua haujlwm.

(2) Thaum cov tshuaj tiv thaiv xa tuaj, nws tseem yuav tsum khaws cia rau hauv lub xeev muaj tub yees, thauj los ntawm cov tub yees txias, thiab ua kom luv luv lub sijhawm xa me nyuam sai npaum li sai tau. Tom qab ncav cuag lub hom phiaj, nws yuav tsum muab tso rau hauv 4 ° C tub yees. Yog tias tsis muaj lub tsheb thauj khoom nyob hauv tub yees, nws yuav tsum thauj nws siv lub hnab yas khov (tshuaj tiv thaiv ua kua) lossis dej khov (txhaj tshuaj qhuav).

(3) Cov tshuaj tiv thaiv kab mob ntawm tes, xws li cov tshuaj tiv thaiv ua kua rau tus tiv thaiv qaib ntxhw-herpesvirus ntawm Marek, yuav tsum muab khaws cia rau hauv cov kua nitrogen li ntawm 195 ° C. Thaum lub sijhawm khaws cia, xyuas seb lub kua nitrogen hauv lub ntim puas ploj txhua lub limtiam. Yog hais tias nws tau hais txog qhov yuav ploj mus, nws yuav tsum tau txais kev pab ntxiv.

(4) Txawm hais tias lub teb chaws pom zoo rau cov tshuaj tiv thaiv uas tsim nyog, yog tias nws khaws tsis raug, thauj thiab siv, nws yuav cuam tshuam rau qhov zoo ntawm cov tshuaj tiv thaiv thiab txo nws cov kev ua tau zoo.

Thib ob, kev siv tshuaj tiv thaiv yuav tsum them sai sai rau cov teeb meem

(1) Ua ntej tshaj plaws, yuav tsum nyeem cov lus qhia siv los ntawm lub chaw tsim tshuaj, thiab raws li nws siv thiab ntau npaum li cas.

(2) Kuaj seb lub raj mis tshuaj tiv thaiv puas muaj daim ntawv pov thawj kev nplaum nplaum thiab seb nws dhau sijhawm tas sijhawm. Yog tias nws tau tshaj lub hnub txhaj tshuaj tag sijhawm, nws siv tsis tau.

(3) Cov tshuaj tiv thaiv yuav tsum zam kiag lub hnub ci.

(4) Cov phwj yuav tsum tau muab rhaub los yog lub zog autoclaved thiab yuav tsum tsis muaj tshuaj tua kab mob (haus dej cawv, stearic acid, thiab lwm yam).

(5) Qhov tshuaj tiv thaiv qhuav tom qab sib ntxiv ntawm cov tshuaj daws tau yuav tsum tau siv sai li sai tau thiab nws yuav tsum siv nyob rau hauv 24 teev ntawm qhov kawg.

(6) Cov tshuaj tiv thaiv yuav tsum tau siv rau hauv cov tsiaj muaj kev noj qab haus huv. Txhaj tshuaj tiv thaiv yuav tsum raug ncua yog tias tsis muaj lub zog, tsis qab los, kub taub hau, raws plab, lossis lwm yam tsos mob. Txwv tsis pub, tsis tsuas yog tsis tuaj yeem tau txais kev tiv thaiv zoo, thiab yuav ua rau kom nws muaj mob ntau dua.

(7) Tshuaj tiv thaiv kev tiv thaiv Feem ntau cov neeg txuas ntxiv tau ntxiv, tshwj xeeb tshaj yog cov roj tau yooj yim rau nag lossis daus. Txhua zaus cov tshuaj tiv thaiv tawm ntawm cov koob txhaj tshuaj, lub raj mis tshuaj tiv thaiv tau tawm los ntawm kev txhaj tshuaj thiab cov ntsiab lus ntawm cov tshuaj tiv thaiv tau ua homogenized kiag li ua ntej siv.

(8) Cov tshuaj tiv thaiv tsis muaj hwj thiab cov tshuaj tiv thaiv tsis siv yuav tsum tau muab tshuaj tua kab mob thiab muab pov tseg.

(9) Sau txhua huv si hom kev txhaj tshuaj uas siv, hom muaj npe, lawb naj npawb, hnub tas sij hawm, hnub txhaj, thiab txhaj tshuaj teb, thiab khaws cia rau yav tom ntej.

Thib peb, kev txhaj tshuaj tiv thaiv dej rau cov qaib yuav tsum them nyiaj rau cov xwm txheej

(1) dej ntws tawm yuav tsum yog dej huv tsis muaj tshuaj ntxuav tais diav tom qab siv tas.

(2) Cov tshuaj tiv thaiv tsis zoo yuav tsum tsis txhob muaj cov tshuaj tua kab mob uas muaj cov tshuaj tua kab mob lossis qee cov kua qaub lossis kua dej. Distilled dej yuav tsum siv. Yog tias koj yuav tsum siv cov kais dej, ntxiv txog 0.01 gram ntawm Hypo (Sodium thiosulfate) rau 1,000 ml ntawm cov kais dej tom qab tshem cov kais dej los ntxuav cov kais dej, lossis siv nws li 1 hmo.

(3) Dej haus yuav tsum tau muab ncua tseg ua ntej inoculation, kwv yees li 1 teev nyob rau lub caij ntuj sov thiab txog 2 teev nyob rau lub caij ntuj no. Nyob rau lub caij ntuj sov, qhov kub ntawm dawb dev mub yog siab heev. Txhawm rau kom txo qhov poob ntawm cov tshuaj tiv thaiv cov tshuaj tiv thaiv, nws raug nquahu kom ua rau cov dej haus tsis haum rau lub cev thaum qhov kub tsawg thaum sawv ntxov.

(4) Tus nqi ntawm cov dej haus hauv cov tshuaj tiv thaiv tsim nyob hauv 2 teev. Tus nqi ntawm cov dej haus rau ib cov kua tauj ib hnub tau muaj raws li hauv qab no: 4 hnub ntawm hnub nyoog 3 ˉ 5 ml 4 lub lis piam ntawm hnub nyoog 30 ml 4 lub hlis ntawm 50 xyoo

(5) Haus cov dej haus ib 1,000 ml Ntxiv 2-4 gram ntawm cov hmoov mis nyuj skimmed kom tiv thaiv cov tshuaj tiv thaiv cov kab mob muaj sia.

(6) Cov chaw haus dej txaus tsim nyog yuav tsum npaj. Tsawg kawg ntawm 2/3 ntawm cov qaib hauv ib pawg qaib tuaj yeem haus dej ntawm tib lub sijhawm thiab ntawm kev cuam tshuam thiab mus deb.

(7) Cov dej haus tsis haum rau cov dej haus yuav tsum tsis txhob ntxiv rau hauv cov dej haus tsis pub dhau 24 teev tom qab kev saib xyuas dej haus. Vim hais tias ntawm impeding lub loj hlob ntawm cov tshuaj tiv thaiv tus kab mob nyob rau hauv qaib.

(8) Txoj kev no yooj yim dua thiab kev siv zog-txuag dua li kev txhaj tshuaj lossis qhov muag, cov nqaij ntawd-qhov ntswg, tab sis kev tsim cov tshuaj tiv thaiv tsis zoo yog nws qhov tsis zoo.

Cov lus 1 Tso cov dej haus kom txaus rau cov dej haus Tus qaib muaj hnub nyoog 4 hnub 14 hnub nyoog 28 hnub qub 21 hnub nyoog Ntev 1,000 noj dej haus 5 liv 10 litres 20 litres 40 litres Nco ntsoov: Nws tuaj yeem nce lossis txo qis raws lub caij. Plaub, kev txhaj tshuaj qaib rau kev tsim txom yuav tsum them nyiaj rau cov teeb meem

(1) txau tshuaj lom yuav tsum raug xaiv los ntawm huv tsev qaib cov qaib kom huv vim yog kev siv cov kua txiv kom zoo, vim cov qauv no piv nrog lub qhov muag, lub qhov ntswg thiab txoj kev haus, muaj mob hnyav ua pa, Yog tias raug kev txom nyem los ntawm CRD CRD zuj zus. Tom qab cov tshuaj tsuag inoculation, nws yuav tsum raug tswj cia hauv kev tswj kev nyiam huv.

(2) Cov npua txau tsim los ntawm kev txau yuav tsum muaj 4 lub lis piam los yog laus dua thiab yuav tsum tau txhaj thawj zaug los ntawm tus neeg uas tau txhaj tshuaj tiv thaiv kab mob tsawg dua.

(3) Dilutions yuav tsum muab tso rau hauv lub tub yees 1 hnub ua ntej inoculation. Ib 1,000 ntsiav tshuaj ntawm dilution tau siv nyob rau hauv tawb ntawm 30 ml thiab tiaj tus pub ntawm 60 ml.

(4) Thaum cov tshuaj tsuag tau tiv thaiv, lub qhov rai, tso tawm kiv cua, thiab lub qhov tso pa tawm yuav tsum raug kaw thiab ib ces kaum ntawm lub tsev yuav tsum mus txog. Nws yog qhov zoo dua los npog daim ntaub yas.

(5) Cov neeg ua haujlwm yuav tsum looj ntsej muag thiab tsom iav qhov cua.

(6) Txhawm rau tiv thaiv tus kabmob ua pa, siv cov tshuaj tua kab mob ua ntej thiab tom qab txau.

Qhov tsib, kev siv cov qaib hauv kev siv tshuaj tiv thaiv

(1) Cov tshuaj tiv thaiv tshiab qaib quail qua tuaj yeem muab faib ua cov tshuaj tiv thaiv nyob thiab tsis muaj tshuaj tiv thaiv.